isolation of ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis
نویسندگان
چکیده
ornithobacterium rhinotracheale (ort) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in mazandaran province. these birds were suspected to pasteurellosis by the farm veterinarian. no other marked gross lesion except emaciation was seen. histopathologic examination of the brains showed mild to moderate meningeal vasculitis, perivascular cuffing with lymphocytes, degeneration and necrosis of purkinje cells in the cerebellum. viral culture of the brains especially for newcastle disease and avian influenza viruses was negative. bacterial culture of the brains onto the blood agar revealed pure growth of ornithobacterium rhinotracheale. in this study molecular confirmation of ort by using of a very specific polymerase chain reaction (pcr) was carried out. amplification products of a 784 bp region of the 16s rrna gene of ort confirmed the bacterium identification. this is the first field case of ort isolation from the brain of commercial chickens in iran. these data suggest that this bacterium should be considered in differential diagnosis in cases of avian nervous signs. further studies are necessary to confirm if ort is a primary pathogen in such cases.
منابع مشابه
Isolation of Ornithobacterium rhinotracheale from the brains of commercial broiler breeder chickens with meningitis and encephalitis
Ornithobacterium rhinotracheale (ORT) has been identified as one of the respiratory bacterial pathogens in turkey and chicken flocks. Four live birds displaying severe torticollis were submitted from a 13-week-old commercial broiler breeder chicken flock located in Mazandaran province. These birds were suspected to pasteurellosis by the farm veterinarian. No other marked gross lesion except ema...
متن کاملCharacterization of Ornithobacterium rhinotracheale Isolates from Commercial Chickens
Ornithohac/erium rhino/rachea/e (ORT) is a relatively rceently diseovered bacterium. which it is associated with respiratory diseases, Tracheal samplcs from carcasses of 100 roultry tlocks submitted to Razi Institute were examined for ORT isolation. Ali these tlocks were affected with respiratory disorders. Colonies of ORT were detected after 24h of incubation and identification of ORT isolates...
متن کاملSeroprevalence and identification of Ornithobacterium rhinotracheale from broiler and broiler breeder flocks in Thailand.
Ornithobacteriosis is an infectious disease of avian species that has been reported in almost all countries around the world, except Thailand. The objectives of this study were to determine the seroprevalence of Ornithobacterium rhinotracheale (ORT) and to isolate and identify ORT in broilers and broiler breeders in Thailand. Chicken antibodies had been randomly checked from 17 farms (19 flocks...
متن کاملOrnithobacterium rhinotracheale and Mycoplasma synoviae in broiler chickens in Jordan.
A cross-sectional study was conducted from November 2008 to July 2010 in commercial broiler flocks in southern (n = 50) and northern (n = 50) areas of Jordan, to determine the flock-level prevalence of Ornithobacterium rhinotracheale (ORT) and Mycoplasma synoviae (MS) infections. Tracheal swabs were collected from commercial broilers with respiratory disease and tested by polymerase chain react...
متن کاملIsolation of Ornithobacterium rhinotracheale from the chickens of a broiler farm in Kermanshah province, west of Iran
On June 2005, a respiratory disease was observed in the chickens of a large broiler farm in Kermanshahprovince, west of Iran. Relatively severe respiratory signs started with sneezing at 27 days of age. Thedisease lasted up to the end of fattening period and accompanied by increased mortality (13.6%). At postmortemexamination, tracheitis, airsacculitis and pneumonia were obvious. Serologic exam...
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عنوان ژورنال:
archives of razi instituteناشر: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
دوره 70
شماره 3 2016
میزبانی شده توسط پلتفرم ابری doprax.com
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